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. 2014 Apr 22;23(17):2046–2066. doi: 10.1089/scd.2013.0373

FIG. 6.

FIG. 6.

Microenvironmental induced alterations in TERT splice variant expression profiles in hESCs cultured in 2% and 20% O2 conditions. hESCs were cultured in feeder-free conditions in either high (20%) or low (2%) O2 and harvested to monitor TERT isoform transcript abundance. (A) A representative agarose gel electrophoresis of mRNA transcript abundance for constitutively spliced (full length) TERT and alternatively spliced TERT variants (Δα, Δβ, and ΔαΔβ) in hESCs grown under 2% and 20% O2 conditions for 5 days. (B–D) Densitometric analysis of splice variant amplicons normalized to β-actin transcript levels. (B) TERT isoform transcript abundance was compared in hESCs cultured under optimal conditions in 2% versus 20% O2 atmospheres. (C, D) TERT isoform transcript abundance was also compared in hESCs cultured under conditions known to maintain (feeding; daily media changes in 2% and 20% O2 for 72 h) or not maintain (nonfeeding; no media changes in 20% O2 for 72 h) hESC proliferation in the undifferentiated cell state. Error bars represent SE, n=3. Letters above the histogram bars indicate significant differences (P<0.05).