FIG. 7.

hTERT splice variant transcripts are associated with polyribosomes in hESCs. (A) Absorbance profile for ribosomes separated on a 10%–50% sucrose gradient. The 40S, 60S, and 80S ribosome subunit peaks and fractions containing light and heavy polysomal peaks are indicated. (B) Relative enrichment of (i) Δβ hTERT isoform, (ii) ΔαΔβ hTERT isoform, and (iii) the nonprotein coding X-inactive specific transcript (XIST) mRNAs relative to input measured by reverse transcription quantitative polymerase chain reaction (RT-qPCR) in the light and heavy polysome fractions and in whole-cell hESC lysates (Input) is plotted (n=2, r=3). Negative, water control samples.