FIG. 5.

Characterization of ESC conversion into cEpiSC. (A) Changes of methylation in the six promoters assessed by bisulfite-sequencing during conversion of ESCs into cEpiSCs. (B) Changes in the CpG level of methylation in the promoter of Aebp1, Chrna3, and Daam2 during the conversion process. Genomic DNA after bisulfite conversion was directly sequenced. Each CpG has been classified according to the presence of a C (methylated), a T (unmethylated), or a polymorphism meaning heterogeneity between the two forms in the cell population (both). (C) Changes in expression of DNA methylation modifying enzymes, during the conversion of ESCs determined by RT-qPCR. Bars represent SEM of three independent experiments. *P<0.05, Mann–Whitney U test.