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. 2014 Apr 16;23(17):2014–2029. doi: 10.1089/scd.2013.0639

FIG. 7.

FIG. 7.

Differential methylation in embryo-derived pluripotent cells and in the epiblast. (A) Methylation status in the epiblast (E6.5). (B) Comparison of methylation in promoters common to Borgel et al. [12]) and our study. Promoters were classified according to their mean methylation status in EpiSCs and each category further separated according to their methylation values in E6.5 epiblasts. (C) Gene expression of DNA methylation modifying enzymes in E6.5 epiblast and EpiSCs determined by RT-qPCR. Bars represent SEM. *P<0.05, Mann–Whitney U test. (D) Changes in the CpG level of methylation in the promoter of Aebp1, Chrna3, and Daam2 during derivation of EpiSCs. Genomic DNA after bisulfite conversion was directly sequenced and the status of the CpGs was indicated as in Figs. 5 and 6. (E) Expression status in the epiblast of genes that contain methylated promoters in EpiSCs.