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. 2014 Apr 24;23(17):2004–2013. doi: 10.1089/scd.2013.0525

FIG. 1.

FIG. 1.

Etv2 and Sox7 expression patterns in embryonic bodies (EBs). (A) Etv2 mRNA expression in EBs prepared using A2lox-Cre mES cells. EBs were cultured and harvested at indicated time points. (B) Sox7 mRNA expression in EBs prepared using A2lox-Cre mES cells. EBs were cultured and harvested at specific time points. Etv2 and Sox7 mRNA expression was determined using qRT-PCR and normalized to Gapdh expression. (C) Sox7 mRNA expression in EBs prepared using mES derived from Etv2-null blastocysts and their wild-type littermates. (D) Etv2 versus Sox7 mRNA expression in single EPCs (n=40) derived from EBs harvested on day 5 and sorted for FLK1+/CDH5+. (E) Schematic of the reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (TRE) dox-inducible Etv2 over-expression system. Dox-inducible ETV2 over-expression for 24 h (day 2–3) was confirmed using western blot analysis. Sox7 mRNA expression was determined in EBs harvested on day 3 by qRT-PCR. Induction of ETV2 revealed significantly increased expression of Sox7 mRNA (*P<0.05, n=3).