FIG. 3.

SOX7 over-expression results in increased levels of endothelial progenitors cells (EPCs) and endothelial gene expression. (A) EBs were prepared using SOX7-MYC over-expressing mES cells and cultured for 6 days. Dox was added for 72 h (days 3–6). SOX7-MYC over-expression was confirmed by western blot analysis. (B) FACS analysis of FLK1⁺/CDH5⁺ double-positive EPCs derived from EBs at day 6 showed a significant increase in SOX7 over-expressing EBs (*P<0.05, n=7). (C) SOX7 over-expression for 72 h (day 3–6) increased expression of the endothelial genes PECAM1 and CDH5 in EBs at day 6 as shown by western blot analysis. (D) Induction of SOX7 with dox for 24 h (days 2–3) in EBs harvested on day 3, before the onset of endogenous Etv2, revealed increased expression of endothelial transcripts by qRT-PCR (*P<0.05, n=3). (E) Induction of ETV2 with dox for 24 h (days 2–3) in EBs harvested on day 3, before the onset of endogenous Etv2, revealed increased expression of endothelial transcripts by qRT-PCR. Note the level of induction is higher with ETV2 (E) compared with SOX7 (D) over-expressing cells.