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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Gene. 2014 Feb 13;539(2):190–197. doi: 10.1016/j.gene.2014.02.012

Fig. 2.

Fig. 2

Effect of suppression of UPF1 by RNA interference on NMD of R1032Gfs*25. A double stable mammalian expression system was used to express hERG minigenes and either scrambled (Con) or UPF1 (UPF1) shRNAs. (A) Immunoblot analysis of UPF1 protein. (B) RPA analysis of the effect of UPF1 knockdown. RPA signals were quantified, normalized to hygromycin resistance gene (Hygro), and shown as percentage of WT control (n=3, ***P<0.001).