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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Gene. 2014 Feb 13;539(2):190–197. doi: 10.1016/j.gene.2014.02.012

Fig. 3.

Fig. 3

Effect of downstream intron on NMD of the R1032Gfs*25 mutation. (A) Diagram of the minigenes of WT, R1032Gfs*25 and R1032Gfs*25 without intron 14. (B) RPA analysis of mRNA from WT, R1032Gfs*25 and R1032Gfs*25 without intron 14 minigenes. Cells were treated (+) or not treated (−) with 100 μg/ml of CHX for three hours before RNA isolation. RPA signals were quantified, normalized to hygromycin resistance gene (Hygro), and shown as percentage of WT control (n=3 or 4, **P<0.01).