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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Immunity. 2014 Aug 14;41(2):207–218. doi: 10.1016/j.immuni.2014.07.010

Figure 1. The T cell receptor dictates commitment to the DN TCRαβ+ T cell lineage.

Figure 1

Flow cytometry analysis of GFP+ cells isolated from the small intestinal epithelium (SI) and the spleen (Spl) from Rag1−/− BM chimera expressing TCR Mathilde, Lucienne, Diego, Ronja or Ninon analysed 28 days post-reconstitution. (A) Graph shows the percentage of T cells among GFP+ cells isolated from the small intestinal epithelium (SI) or spleen (Spl). (B) Panels represent the percentage of CD4+, CD8αβ+ or CD4− CD8αβ− (DN, double negative) cells gated on GFP+ TCRαβ+ T cells isolated from the small intestine or spleen as in B. For comparison, the percentage of CD4+, CD8αβ+ or DN cells gated on TCRαβ+ αGalCer-CD1d-tetramer − T cells in B6 is shown. Data points are shown for each mouse, with mean plus s.e.m., from at least three independent experiments (TCR Mathilde (n=8), Lucienne (n=10), Diego (n=9), Ronja (n=6), Ninon (n=8) and C57BL/6 (n=6)). (See also Figure S1)