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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Immunity. 2014 Aug 14;41(2):207–218. doi: 10.1016/j.immuni.2014.07.010

Figure 3. DN TCRαβ+ thymocyte precursors have a mature post-selected phenotype.

Figure 3

(A) Representative flow cytometry analysis of GFPhi thymocytes from chimeras expressing TCR Mathilde analyzed 28 days post reconstitution. CD4 and CD8α profile of GFPhi cells (top panel); TCRβ and TCR Vα2 profile of gated DN thymocytes (bottom left). Expression of CD69, PD1, CD5 and CD122 on gated DN TCRαβ+ thymocytes is depicted (bottom middle and left panels). (B) Graphs represent percentage of GFP+ DN TCRαβ+ thymocytes expressing CD69, Thy1.2, CD5, CD122 and PD1 in chimeras expressing TCR Mathilde (n=8), TCR Lucienne (n=10), TCR Diego (n=9), TCR Ronja (n=6) or TCR Ninon (n=8). Data points are shown for each mouse, with mean plus s.e.m. from at least two independent experiments with a minimum of two mice per experiment. (See also Figure S4)