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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Immunity. 2014 Aug 14;41(2):207–218. doi: 10.1016/j.immuni.2014.07.010

Figure 6. A subset of DN TCRαβ intraepithelial T cells is restricted to non classical MHC Class I.

Figure 6

(A-F) Frequency of DN TCRαβ+ cells in GFP+ gated intraepithelial T cells isolated from various MHC deficient chimeras expressing each individual TCR clone and analysed five weeks after reconstitution. Data points are shown for each mouse from at least two independent experiments, with mean plus s.e.m. (A) Analysis of chimera expressing TCR Diego (B6 (n=4), B2m−/− (n=5), H2-Kb−/−Db−/− (n=5), H2-T3b−/− (n=5), H2-T23−/− (n=4) and Mr1−/− (n=4). (B) Analysis of chimera expressing TCR Ronja (B6 (n=4), B2m−/− (n=5), H2-Kb−/−Db−/− (n=4), H2-T3b−/− (n=4), H2-T23−/− (n=4), and Mr1−/− (n=4)). (C) Analysis of chimera expressing TCR Ninon (B6 (n=6), B2m−/− (n=5), H2-Kb−/−Db−/− (n=6), H2-T3b−/− (n=5), Cd1d1−/− Cd1d2−/− (n=4), H2-T23−/− (n=10) and Mr1−/− (n=6)). (D) Analysis of chimera expressing TCR Diego (B6 (n=4), BALB/cJ (n=4), BALB/cByJ (n=4), and B6.C-H2d/bByJ (n=6)), TCR Ronja (B6 (n=4), BALB/cJ (n=6), BALB/cByJ (n=6), and B6.C-H2d/bByJ (n=4)) and TCR Ninon (B6 (n=6), BALB/cJ (n=4), BALB/cByJ (n=5), and B6.C-H2d/bByJ (n=7)). (E) Analysis of B2m−/− BM chimera for TCR Diego (n=4), TCR Ronja (n=5) and TCR Ninon (n=4). Analysis of Tap1−/− BM chimera for TCR Diego (n=4), TCR Ronja (n=4) and TCR Ninon (n=5)). (F) Analysis of chimera expressing TCR Ninon from at least two independent experiments (B6 (n=6), B2m−/− (n=5), Cd1d1−/− Cd1d2−/− (n=4), Fcgrt−/− (n=7) and Hfe−/− (n=4). A two-tailed Mann-Whitney test was performed. * P<0.05; ** P <0.01; *** P <0.001; and NS, not significant. (See also Figure S6)