Table 4.
Survival of Motor Neuron Reporter Assays
| Assay type | Mechanistic target | Reporter | Host cells | Compounds | Ref. |
|---|---|---|---|---|---|
| Promoter | SMN2 promoter | β-Lactamase | NSC-34 | Diaminoquinazolines | 84 |
| SMN1 promoter | SEAP | 16.4 cells | Taxol | 78 | |
| Splicing | Splicing cassette | Luciferase | C33a | Sodium vanadate | 93 |
| Indoprofen | 92 | ||||
| NSC-34 | Aclarubicin | 169 | |||
| Stability | Read-through | Renilla luciferase | AD293 | G418 | 181 |
| Degron | Luciferase | HEK293 | — | 183 | |
| Multifunction | SMN2 promoter/splicing cassette/stability | Luciferase | HEK293 | 4-arylthiazolyl piperidines | 89 |
| Heterocyclic carboxamides | 33 | ||||
| Dihydroquinolones | 33 | ||||
| Viability | Cellular proliferation | Cell numbers | NIH3T3 | — | 90 |
SEAP, secreted alkaline phosphatase.