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. 2014 Sep 10;21(8):1156–1163. doi: 10.1089/ars.2014.5888

FIG. 2.

FIG. 2.

Inflammation, redox-related gene expression, and antioxidant enzymes in atrial myocardium. Representative immunoblots of (A), NFκB-p65, and (B) Nrf2 in nuclear extracts prepared from 4 individual Ctl and n-3 PUFA-treated patients, with TATA-binding protein (TATA-BP) as loading control. Expression of genes known to be activated by NFκB (C), and those activated by Nrf2 (D) are shown for both treatment groups. Activity of antioxidant enzymes in atrial homogenate from both treatment groups is shown for (E), Catalase, (F), glutathione peroxidase (GPx), (G), glutathione reductase (GR), and (H), total GSH (GSHt). Gene expression data are reported as mean±SD. Raw data are shown for enzyme activity and GSHt in both treatment groups, along with the mean (faint horizontal line). N=10–12 for each group. *p<0.05 versus Ctl, **p<0.01 versus Ctl.