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. 2014 Jul 21;25(4):241–252. doi: 10.1089/hgtb.2013.149

FIG. 1.

FIG. 1.

Transposon constructs and their application in studying overproduction inhibition on the level of excision of DNA transposons. (A) Schematic representation of the four DNA transposons used. IRDR-L/-R, inverted repeat-direct repeat sequences of SB and PB transposons (left and right); PGK, phosphoglycerate kinase promoter; SV40, simian virus 40 early promoter; CAG, CMV enhancer–chicken β-actin–rabbit β1-globin promoter; pA, polyadenylation signal. (B–D) Transposase dose dependence of excision efficiency: when using lipid-based transfection in (B) HEK-293 cells or (C) HeLa cells, or (D) when using nucleofection in HEK-293 cells. (E) Effect of the amount of transposase on the excision efficiency in the case of different SB and PB variants in HEK-293 cells.