Fig. 1.
Dynamic mass redistribution (DMR) responses of urotensin II (UII). (A) DMR response by 100 nM UII at various HEK293UT cell densities in 384-well plates (3,000–30,000 cells/well). (B) Real-time kinetic responses of UII at various concentrations (0–100 nM) in HEK293UT cells. The final concentrations of UII were indicated in the graph: (◯) 0 nM, (●) 0.1 nM, (△) 0.3 nM, (▲) 1 nM, (▽) 3 nM (▼) 10 nM, (□) 30 nM, and (■) 100 nM. (C) The DMR amplitudes of UII as a function of UII concentration. (D) No DMR response in parental HEK293 cells. All concentration responses represent the mean±SD of triplicate wells (n=3).