Fig. 6.
Calcium profiles of SB657510 (A), GSK1562590 (B), and urantide (C) as reference compounds in HEK293UT cells. The cells in individual wells were incubated with the reference compounds at various concentrations for 120 s after taking a 20 s DMR baseline measurement [SB657510: (◯) 0 μM, (●) 0.156 μM, (△) 0.312 μM, (▲) 0.625 μM, (▽) 1.25 μM, (▼) 2.5 μM, (□) 5 μM, and (■) 10 μM; GSK1562590: (◯) 0 μM, (●) 0.016 μM, (△) 0.031 μM, (▲) 0.063 μM, (▽) 0.125 μM, (▼) 0.25 μM, (□) 0.5 μM, and (■) 1 μM; urantide: (◯) 0 μM, (●) 0.001 μM, (△) 0.01 μM, (▲) 0.1 μM, (▽) 1 μM]. All concentration responses represent the mean±SD of triplicate wells (n=3).