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. 2014 Jul 28;111(33):12264–12269. doi: 10.1073/pnas.1410622111

Fig. 5.

Fig. 5.

Nonconcordantly regulated 10-kb lncRNA-mRNA partners in embryonic hearts. (A) (Left) Quantitative expression of the nonconcordantly regulated lncRNA/mRNA pair n411949/Mccc1 (black is lncRNA; red is mRNA) in embryonic and adult hearts; expression in sham and 4-wk TAC hearts is shown for comparison (differences with TAC do not meet significance criteria). (Right) Relative positions of lncRNA n411949 and mRNA Mccc1 within the genome; other transcripts within the locus are colored blue. (B) (Left) Positions targeted by anti-lncRNA gapmers, and primer design for lncRNA (arrows) and mRNA (arrows + internal probe) qPCR detection. (Right) lncRNA and mRNA expression in C2C12 cells transfected with anti-lncRNA gapmers, relative to geometric mean of Actb, Gapdh, and Hmbs (mean ± SEM, n = 6; representative of at least two independent experiments). Non, nontransfected cells; Neg, transfected with fluorescein amidite (FAM)-labeled control gapmer; gapmeR-1 and -2, transfected with one of two different gapmeRs against the chosen lncRNA. *P < 0.05 relative to negative control (Neg). (C and D) Same as A and B, but for the nonconcordantly regulated lncRNA/mRNA pair n413445/Relb.