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Glutathionylated proteins are released by LPS-stimulated RAW264 cells. Supernatants from BioGEE-preloaded cells exposed to 100 ng/mL LPS for 24 h were analyzed by Western blot analysis with streptavidin peroxidase. Left lane, nonreduced sample; right lane, sample reduced with 10 mM DTT before loading on the gel.
