Fig. 5.

hPRDX2 induces TNF-α production. (A) hrPRDX2 was added at 10 µg/mL to RAW264 cells, and TNF-α production was measured 24 h later by ELISA in triplicate samples. Solid bars represent PRDX2 preincubated with polymixin B, as described in the text; open bars represent PRDX2 preincubated without polymyxin B. (B) TNF-α induction by hrPRDX2 in RAW cells pretreated with 100 U/mL IFN-γ or not pretreated. *P < 0.01 vs. control (A) or vs. PRDX2 alone (B). (C) hrPRDX2 was added to human primary macrophage cultures from four donors, and TNF-α production was measured by ELISA 24 h later in triplicate.