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. 2014 Aug 4;111(33):12097–12102. doi: 10.1073/pnas.1302703111

Fig. 3.

Fig. 3.

HCs contribute to the full osteoblast lineage revealed by tam-inducible lineage tracing. (A and B) X-Gal staining of C10creERt::RLacZ tibiae sections at E15.5 and E18.5 and postnatal stages (P5, P1m) after tam injection at E13.5. In B, LacZ+ cells were found to express OSX, Col1a1, and SOST. (Scale bar, 200 μm.) (C) The proportion of tagged HCs [HC-LacZ+/HCs (%)] in the HZ and tagged Col1a1-expressing cells [Col1a1+;LacZ+/Col1a1+ (%)] in fetal and postnatal ossification centers. Tam was injected at E13.5, and tibiae were X-Gal stained at E16.0, E18.5, and P5 (n = 5 for each stage). Col1a1-expressing cells (by in situ hybridization) in the trabecular bone and endosteum were counted. DP, diaphysis; PO, primary ossification center; TB, trabecular region.