Figure 5.

ERG response following suppression and replacement of P347S RHO. At postnatal day 5, mice were injected in one eye with a 1:30 mixture of AAV expressing the shRNA (S) and AAV expressing the siRNA-resistant replacement gene (R). Contralateral eyes were injected with a control virus expressing EGFP. Six weeks or 20 wk later, mice were dark-adapted overnight and ERG responses recorded from both eyes. (A,B) Overlays of the ERG recordings; gray and black lines represent recordings from combined suppression and replacement therapy (S+R) and control (Ctr)-injected eyes, respectively. RE, right eyes; LE, left eyes. (C) Mean b-wave amplitudes. Gray and black columns represent values corresponding to S+R and control-injected eyes, respectively. Error bars represent standard deviation [*** p < 0.001]. (From Millington-Ward et al. 2011; reprinted, with permission, from Nature Publishing Group © 2011.)