Figure 3. Overview of NAPPA slide production.

APTES-coated glass slides are used to print a mixture of open-reading frame containing plasmids admixed with anti-tag capture antibody, BSA, and BS3 cross-linker. To create the protein array, the slide is treated with coupled in vitro transcription/translation lysate which transcribes RNA leading to localized protein production and local capture. Thereafter, the slides can be utilized for protein-interaction analysis or screening for binding with antibodies in clinical samples.