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. Author manuscript; available in PMC: 2014 Aug 25.
Published in final edited form as: J Steroid Biochem Mol Biol. 2010 Sep 8;122(5):326–332. doi: 10.1016/j.jsbmb.2010.08.007

Fig. 3.

Fig. 3

Cdc25A siRNA (siRNA) reduces ET-dependent Cdk2 activity and DNA synthesis in the adult VSMC. A: Cdc25A siRNA reduces ET-stimulated Cdc25A protein expression. B: Cdc25A siRNA and 10−8 mol/L 1,25(OH)2D individually reduce Cdk2 activity but the combination failed to display additivity. Cdk2 activity was normalized to Cdk2 protein level. Representative blot is presented above the bar graph. C: Treatment with Cdc25A siRNA results in a significant reduction in DNA synthesis. The reduction was not additive with 10−8 mol/L 1,25(OH)2D, included for the 48 hours of treatment. All control groups were treated with siRNA containing scrambled sequence. Pooled data came from 3-5 separate experiments. *P<0.01 vs. ET alone; +P<0.01, #P<0.05 vs. control.