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. Author manuscript; available in PMC: 2014 Aug 25.
Published in final edited form as: J Steroid Biochem Mol Biol. 2010 Sep 8;122(5):326–332. doi: 10.1016/j.jsbmb.2010.08.007

Fig. 4.

Fig. 4

1,25(OH)2D inhibits AII-induced DNA synthesis, Cdc25A gene expression and Cdk2 activity. A: After transfection with Cdc25A siRNA or scrambled siRNA sequence (C) for 24 hours, cells were treated with 10−8 mol/L 1,25(OH)2D or vehicle for 48 hours. Where indicated, AII (10−7 mol/L) was included for the final 24 hours prior to measurement of 3H-thymidine incorporation. B: Cells were exposed to 10−8 mol/L 1,25(OH)2D for 48 hours and AII for the last 9 hours of the incubation prior to measurement of Cdc25A gene expression. Cdc25A mRNA levels were measured by real-time PCR and normalized to 18S rRNA. C: After treatment, as described in Panel A, cells were lysed, immune complex kinase assays and Western blot (WB) analyses for Cdk2 were carried as described in Methods. Cdk2 activity was normalized to Cdk2 protein levels. Representative results and pooled data are shown. Experiments were repeated 3-4 times. *P<0.01 or **P<0.05 vs. AII alone. +P<0.01 vs. control.