TABLE I.
Oligonucleotides used in the construction of sDQ2-peptide and sDR3-CLIP1 complexes.
| CLIP1 oligonucleotides | Fwd | 5'-CCGGACCTGTGAGCAAGATGAGAATGGCCACCCCCCTGCTCATGCAGGCTGG-3' |
| Rev | 5'-CCGGAATGGCCACCCCCCTCCTTATGCAGGCACTGCCTATGGGCGCTCTGGG-3' | |
| CLIP2 oligonucleotides | Fwd | 5'-CGCCAGCCTGCATGAGCAGGGGGGTGGCCATTCTCATCTTGCTCACAGGT-3' |
| Rev | 5'-CGCCCAGAGCGCCCATAGGCAGTGCCTGCATAAGGAGGGGGGTGGCCATT-3' | |
| CLIP1 M91A mutagenesis primer | Fwd | 5'-CCTGTGAGCAAGGCGAGAATGGCCACCCC-3' |
| CLIP1 M91P mutagenesis primer | Fwd | 5'-CCTGTGAGCAAGCCGAGAATGGCCACCCC-3' |
| DRα amplification primers | Fwd | 5'-GAAGATCTATGGCCATAAGTGGAGTC-3' |
| Rev | 5'-ATAATGATGCCCACCAGACC-3' | |
| DRβ amplification primers | Fwd | 5'-CGGCGGAGGCGGTAGTGGGGACACCAGACCACGTTT-3' |
| Rev | 5'-CCGCGACCTTCAATGTCTACCTTGCTCTGTGCAGATTCAG-3' | |
| Fos amplification primers | Fwd | 5'-TCTCCCAGAGACTACAGAGAACGTGGACATCGAAGGACGTGG-3' |
| Rev | 5'-GAAGATCTTCAGGCGGCCAGGATG-3' | |
| Jun amplification primers | Fwd | 5'-GTAGACATTGAAGGTCGCGG-3' |
| Rev | 5'-GAGGATCCTTAGTTGTGCCATTCT-3' | |
| DQB1*0201-CLIP1+LS amplification primers | Fwd | 5'-GAGGATCCATGTCCTGGAAAAAGG-3' |
| Rev | 5'-ACTACCGCCTCCGCCG-3' |