Figure 6. Effects of anti-TNF-α, anti-IL-6, and anti-IL-10 treatment on hypoxia-inducible factor-1α (HIF-1α) expression in skeletal muscle ischemia-reperfusion (IR) injury that occurs after 3 h ischemia and 3 h reperfusion. A, Quantification of HIF-1α expression. B, Effect of anti-TNF-α on the expression of HIF-1α. C, Effect of anti-IL-6 on the expression of HIF-1α. D, Effect of anti-IL-10 on the expression of HIF-1α. Data are reported as means±SD. ^⋆P<0.05 vs sham-operated group (C); ^▾P<0.05 vs IR group (one-way ANOVA).