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Unsupervised hierarchical clustering of top 30 ranked candidate metabolites (columns) and samples (rows) is performed, and a heat map was generated. “N”, “T” and “M” represented the benign, clinically localized prostate cancer, or metastatic cancer samples, respectively. The top 30 ranked metabolites are from (A) KEGG metabolic network and (B) EHMN metabolic network.
