Table 4. EST-SSR primers used, number of alleles per locus, allele size range, polymorphic information content (PIC) and allelic richness for each genome.
PIC | Allelic richness | |||||||||||||||||||||||
Genome number | Genome number | |||||||||||||||||||||||
Primer | No. of alleles | Allele size range (bp)† | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Overall | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Overall |
VES0019 | 16 | 253–283 (30) | 0.45 | 0.00 | 0.21 | 0.77 | 0.81 | 0.00 | 0.07 | 0.40 | 0.85 | 0.61 | 0.77 | 3.6 | 1.0 | 2.0 | 5.9 | 6.2 | 1.0 | 1.5 | 2.8 | 7.2 | 3.6 | 5.8 |
VES0021 | 18 | 217–289 (72) | 0.46 | 0.00 | 0.00 | 0.51 | 0.59 | 0.00 | 0.00 | 0.13 | 0.37 | 0.04 | 0.75 | 3.0 | 1.0 | 1.0 | 3.6 | 4.1 | 1.0 | 1.0 | 1.8 | 3.0 | 1.3 | 5.3 |
VES0070 | 7 | 265–275 (10) | 0.00 | 0.00 | 0.00 | 0.50 | 0.46 | 0.00 | 0.07 | 0.64 | 0.17 | 0.00 | 0.61 | 1.0 | 1.0 | 1.0 | 3.3 | 2.7 | 1.0 | 1.5 | 3.8 | 2.0 | 1.0 | 3.5 |
VES0093 | 11 | 183–199 (16) | 0.00 | 0.00 | 0.00 | 0.51 | 0.15 | 0.00 | 0.00 | 0.66 | 0.05 | 0.40 | 0.75 | 1.0 | 1.0 | 1.0 | 3.3 | 1.9 | 1.0 | 1.0 | 3.9 | 1.3 | 2.3 | 5.0 |
VES0116 | 4 | 278–284 (6) | 0.13 | 0.37 | 0.00 | 0.00 | 0.00 | 0.13 | 0.00 | 0.13 | 0.00 | 0.22 | 0.36 | 1.8 | 2.0 | 1.0 | 1.0 | 1.0 | 1.7 | 1.0 | 1.8 | 1.0 | 1.9 | 2.2 |
VES0120 | 16 | 245–293 (48) | 0.13 | 0.00 | 0.00 | 0.16 | 0.52 | 0.00 | 0.10 | 0.73 | 0.00 | 0.00 | 0.51 | 1.8 | 1.0 | 1.0 | 2.0 | 4.2 | 1.0 | 1.6 | 4.8 | 1.0 | 1.0 | 3.5 |
VES0202 | 15 | 218–254 (36) | 0.55 | 0.00 | 0.00 | 0.58 | 0.24 | 0.00 | 0.00 | 0.43 | 0.46 | 0.00 | 0.71 | 4.4 | 1.0 | 1.0 | 3.4 | 2.1 | 1.0 | 1.0 | 2.9 | 2.9 | 1.0 | 4.6 |
VES0204 | 20 | 310–366 (56) | 0.61 | 0.00 | 0.00 | 0.69 | 0.70 | 0.10 | 0.24 | 0.44 | 0.21 | 0.31 | 0.71 | 4.5 | 1.0 | 1.0 | 4.7 | 5.4 | 1.6 | 2.6 | 2.8 | 2.4 | 2.5 | 5.0 |
VES0335 | 15 | 265–284 (19) | 0.44 | 0.00 | 0.21 | 0.78 | 0.29 | 0.04 | 0.40 | 0.00 | 0.60 | 0.55 | 0.81 | 2.8 | 1.0 | 2.0 | 6.4 | 2.3 | 1.3 | 2.7 | 1.0 | 3.7 | 3.6 | 6.5 |
VES0427 | 9 | 316–329 (13) | 0.50 | 0.32 | 0.00 | 0.26 | 0.47 | 0.00 | 0.00 | 0.34 | 0.16 | 0.31 | 0.71 | 3.0 | 2.0 | 1.0 | 2.1 | 3.3 | 1.0 | 1.0 | 2.8 | 1.8 | 2.4 | 4.5 |
VES0478 | 11 | 298–309 (11) | 0.72 | 0.00 | 0.00 | 0.22 | 0.21 | 0.13 | 0.13 | 0.50 | 0.09 | 0.08 | 0.66 | 5.4 | 1.0 | 1.0 | 1.9 | 1.9 | 1.7 | 1.7 | 3.3 | 1.6 | 1.5 | 4.3 |
VES0546 | 26 | 431–476 (45) | 0.63 | 0.00 | 0.00 | 0.32 | 0.84 | 0.00 | 0.37 | 0.48 | 0.58 | 0.48 | 0.85 | 4.4 | 1.0 | 1.0 | 2.9 | 7.2 | 1.0 | 3.2 | 3.0 | 3.0 | 2.9 | 7.5 |
VES0624 | 4 | 265–271 (6) | 0.00 | 0.00 | 0.00 | 0.00 | 0.15 | 0.04 | 0.00 | 0.00 | 0.00 | 0.00 | 0.42 | 1.0 | 1.0 | 1.0 | 1.0 | 1.9 | 1.3 | 1.0 | 1.0 | 1.0 | 1.0 | 2.6 |
VES0665 | 9 | 195–213 (18) | 0.26 | 0.00 | 0.00 | 0.31 | 0.35 | 0.00 | 0.00 | 0.67 | 0.13 | 0.24 | 0.68 | 2.6 | 1.0 | 1.0 | 2.7 | 2.8 | 1.0 | 1.0 | 5.0 | 1.9 | 2.3 | 4.2 |
VES0670 | 6 | 99–114 (15) | 0.44 | 0.00 | 0.00 | 0.56 | 0.20 | 0.00 | 0.00 | 0.00 | 0.38 | 0.08 | 0.71 | 2.8 | 1.0 | 1.0 | 3.8 | 2.1 | 1.0 | 1.0 | 1.0 | 3.1 | 1.5 | 4.4 |
VES0678 | 7 | 293–318 (25) | 0.45 | 0.00 | 0.00 | 0.40 | 0.54 | 0.00 | 0.00 | 0.36 | 0.28 | 0.41 | 0.70 | 2.9 | 1.0 | 1.0 | 2.3 | 3.3 | 1.0 | 1.0 | 2.0 | 2.0 | 2.5 | 4.5 |
VES0679 | 18 | 310–365 (55) | 0.44 | 0.00 | 0.00 | 0.40 | 0.78 | 0.31 | 0.14 | 0.63 | 0.61 | 0.74 | 0.81 | 2.8 | 1.0 | 1.0 | 3.2 | 5.6 | 2.0 | 1.9 | 4.4 | 3.9 | 5.2 | 7.8 |
VES0749 | 15 | 208–236 (28) | 0.29 | 0.00 | 0.00 | 0.60 | 0.27 | 0.00 | 0.00 | 0.62 | 0.09 | 0.43 | 0.79 | 2.0 | 1.0 | 1.0 | 3.8 | 2.3 | 1.0 | 1.0 | 4.4 | 1.6 | 2.5 | 5.8 |
VES0762 | 7 | 242–266 (24) | 0.13 | 0.00 | 0.21 | 0.62 | 0.08 | 0.00 | 0.04 | 0.37 | 0.00 | 0.00 | 0.43 | 1.8 | 1.0 | 2.0 | 3.6 | 1.5 | 1.0 | 1.3 | 2.0 | 1.0 | 1.0 | 3.0 |
VES0777 | 3 | 166–175 (9) | 0.00 | 0.00 | 0.00 | 0.36 | 0.40 | 0.00 | 0.10 | 0.18 | 0.14 | 0.00 | 0.37 | 1.0 | 1.0 | 1.0 | 2.0 | 2.3 | 1.0 | 1.6 | 1.9 | 1.7 | 1.0 | 2.0 |
VES0803 | 12 | 292–308 (16) | 0.00 | 0.00 | 0.00 | 0.64 | 0.71 | 0.00 | 0.10 | 0.29 | 0.41 | 0.00 | 0.80 | 1.0 | 1.0 | 1.0 | 4.8 | 4.6 | 1.0 | 1.6 | 2.0 | 2.6 | 1.0 | 6.3 |
VES0868 | 12 | 213–288 (75) | 0.13 | 0.00 | 0.00 | 0.53 | 0.27 | 0.00 | 0.00 | 0.26 | 0.35 | 0.00 | 0.62 | 1.8 | 1.0 | 1.0 | 4.1 | 2.2 | 1.0 | 1.0 | 2.6 | 2.0 | 1.0 | 4.2 |
VES0987 | 9 | 298–310 (12) | 0.26 | 0.21 | 0.00 | 0.55 | 0.36 | 0.00 | 0.00 | 0.13 | 0.31 | 0.59 | 0.73 | 2.6 | 2.0 | 1.0 | 3.4 | 3.0 | 1.0 | 1.0 | 1.8 | 2.0 | 3.0 | 5.0 |
VES1001 | 10 | 227–250 (23) | 0.29 | 0.00 | 0.00 | 0.39 | 0.55 | 0.00 | 0.00 | 0.64 | 0.00 | 0.00 | 0.65 | 2.5 | 1.0 | 1.0 | 2.7 | 3.6 | 1.0 | 1.0 | 3.9 | 1.0 | 1.0 | 3.9 |
VES1020 | 6 | 170–183 (13) | 0.26 | 0.00 | 0.00 | 0.48 | 0.51 | 0.07 | 0.07 | 0.00 | 0.00 | 0.37 | 0.73 | 2.6 | 1.0 | 1.0 | 2.9 | 3.3 | 1.5 | 1.5 | 1.0 | 1.0 | 2.0 | 4.6 |
VES1023 | 7 | 140–157 (17) | 0.00 | 0.00 | 0.00 | 0.28 | 0.56 | 0.00 | 0.10 | 0.00 | 0.37 | 0.00 | 0.59 | 1.0 | 1.0 | 1.0 | 2.2 | 3.7 | 1.0 | 1.6 | 1.0 | 2.2 | 1.0 | 3.4 |
VES1029 | 9 | 111–128 (17) | 0.58 | 0.00 | 0.00 | 0.62 | 0.04 | 0.00 | 0.00 | 0.00 | 0.52 | 0.00 | 0.61 | 3.8 | 1.0 | 1.0 | 3.8 | 1.3 | 1.0 | 1.0 | 1.0 | 2.9 | 1.0 | 3.9 |
VES1067 | 14 | 447–477 (30) | 0.13 | 0.00 | 0.37 | 0.68 | 0.60 | 0.00 | 0.32 | 0.36 | 0.17 | 0.37 | 0.83 | 1.8 | 1.0 | 2.0 | 4.5 | 3.9 | 1.0 | 2.8 | 2.0 | 2.1 | 2.0 | 6.7 |
VES1082 | 14 | 281–317 (36) | 0.40 | 0.00 | 0.00 | 0.51 | 0.70 | 0.00 | 0.00 | 0.34 | 0.13 | 0.46 | 0.70 | 2.8 | 1.0 | 1.0 | 3.1 | 4.8 | 1.0 | 1.0 | 2.8 | 1.9 | 2.8 | 5.0 |
VES1085 | 13 | 390–403 (13) | 0.00 | 0.00 | 0.00 | 0.33 | 0.60 | 0.31 | 0.13 | 0.00 | 0.50 | 0.74 | 0.82 | 1.0 | 1.0 | 1.0 | 2.6 | 4.1 | 2.5 | 1.7 | 1.0 | 2.8 | 4.7 | 6.6 |
VES1172 | 34 | 217–268 (51) | 0.71 | 0.53 | 0.37 | 0.85 | 0.89 | 0.58 | 0.82 | 0.60 | 0.41 | 0.59 | 0.94 | 6.0 | 3.0 | 2.0 | 7.4 | 9.0 | 3.8 | 6.5 | 3.8 | 2.9 | 4.3 | 9.9 |
VES1196 | 5 | 203–215 (12) | 0.00 | 0.00 | 0.00 | 0.30 | 0.15 | 0.00 | 0.00 | 0.00 | 0.09 | 0.00 | 0.38 | 1.0 | 1.0 | 1.0 | 2.5 | 1.9 | 1.0 | 1.0 | 1.0 | 1.5 | 1.0 | 2.1 |
VES1231 | 6 | 120–139 (19) | 0.23 | 0.00 | 0.00 | 0.53 | 0.08 | 0.00 | 0.00 | 0.00 | 0.34 | 0.00 | 0.52 | 2.0 | 1.0 | 1.0 | 2.9 | 1.5 | 1.0 | 1.0 | 1.0 | 2.6 | 1.0 | 3.5 |
VES1258 | 10 | 369–398 (29) | 0.54 | 0.00 | 0.00 | 0.22 | 0.59 | 0.04 | 0.00 | 0.00 | 0.50 | 0.48 | 0.76 | 3.0 | 1.0 | 1.0 | 1.9 | 3.6 | 1.3 | 1.0 | 1.0 | 2.9 | 3.0 | 5.5 |
VES1263 | 8 | 305–320 (15) | 0.13 | 0.00 | 0.00 | 0.00 | 0.43 | 0.07 | 0.00 | 0.56 | 0.00 | 0.30 | 0.68 | 1.8 | 1.0 | 1.0 | 1.0 | 2.7 | 1.5 | 1.0 | 3.6 | 1.0 | 2.0 | 4.4 |
VES1271 | 3 | 307–310 (3) | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.05 | 0.37 | 0.42 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.3 | 2.0 | 2.6 |
VES1310 | 7 | 288–309 (21) | 0.23 | 0.00 | 0.00 | 0.37 | 0.25 | 0.00 | 0.10 | 0.60 | 0.60 | 0.30 | 0.55 | 2.0 | 1.0 | 1.0 | 3.0 | 2.5 | 1.0 | 1.6 | 3.8 | 3.6 | 2.0 | 3.3 |
VES1469 | 29 | 128–178 (50) | 0.00 | 0.21 | 0.21 | 0.82 | 0.79 | 0.17 | 0.04 | 0.61 | 0.64 | 0.66 | 0.86 | 1.0 | 2.0 | 2.0 | 6.5 | 6.1 | 2.0 | 1.3 | 3.8 | 5.3 | 5.0 | 7.7 |
Total | 445 | |||||||||||||||||||||||
Average | 11.7 | 0.28 | 0.04 | 0.04 | 0.44 | 0.42 | 0.05 | 0.09 | 0.32 | 0.28 | 0.27 | 0.67 | 2.43 | 1.16 | 1.16 | 3.24 | 3.34 | 1.27 | 1.53 | 2.49 | 2.33 | 2.15 | 4.75 |
Difference between the largest and smallest fragments amplified by each primer is shown in parentheses.
See Table 3 for the abbreviations of genome number.