Figure 2. Overexpression of pkcA suppresses the phenotype defects caused by the cnaA deletion.

(A) The wild-type, alcA::pkcA, ΔcnaA and alcA::pkcA ΔcnaA strains were grown for 72 hours at 37°C on agar plates containing either 4% glucose, 2% glycerol or 2% glycerol plus 100 mM threonine, or (B) in liquid media containing 2% glycerol plus 100 mM threonine for 12, 24 and 48 hours at 37°C, before their growth was assessed under the microscope (scale bars indicate 5 µm) and (C) mycelial dry weight measured for three biological replicates from each collected timepoint.