Figure 4. Overexpression of pkcA in a ΔcnaA background rescues aberrant cell wall deposition in a ΔcnaA background.

Wild-type, ΔcnaA and alcA::pkcA ΔcnaA strains were grown in minimal medium supplemented with 2% (w/v) glycerol plus 100 mM threonine for 16 hours at 30°C before being fixed for 30 minutes at room temperature (RT) and stained with fluorescein isothiocyanate wheat germ agglutinin (FITC-WGA) for 5 minutes at RT. Mycelial fluorescence was then assessed under the microscope (scale bars indicate 5 µm).