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. 2014 Aug 25;9(8):e104792. doi: 10.1371/journal.pone.0104792

Figure 5. Overexpression of pkcA in a ΔcnaA background restores the levels of phosphorylated MpkA.

Figure 5

Western blots (A) of the protein crude extract from the wild-type, ΔcnaA, alcA::pkcA and alcA::pkcA ΔcnaA strains, when grown in 2% glycerol plus 100 mM threonine for 16 hours at 30°C before being treated with Congo Red (CR) for 1 hour. Phosphorylated MpkA (49 kDa band) was probed for with anti-MpkA antibodies (B) and signal intensities were quantified using the Image J software by dividing the intensity of Western band by a the Coomassie stained protein bands. kDa, kilo Daltons. These results are from a single representative experiment from three different repetitions that provided comparable results.