Figure 8. Overexpression of pkcA in a ΔcnaA background restores cytochrome c expression.

(A) Western blots of the protein extracts from the wild-type, ΔcnaA, alcA::pkcA and alcA::pkcA ΔcnaA strains when grown in the presence of 2% glycerol plus 100 mM threonine for 16 hours at 37°C. Signal intensities were quantified using the Image J software by dividing the intensity of Western band of the cytochrome c by the Coomassie stained protein bands (B). These results are from a single representative experiment from three different repetitions that provided comparable results.