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. 2014 Aug 25;9(8):e104792. doi: 10.1371/journal.pone.0104792

Figure 10. Overexpression of pkcA in a ΔcnaA background restores oxygen consumption.

Figure 10

(A) Wild-type, ΔcnaA, alcA::pkcA and alcA::pkcA ΔcnaA germlings were grown in minimal medium supplemented with 2% glycerol plus 100 mM threonine for 16 hours at 37 C and stained with nonyl acridine orange for 10 minutes at 37°C. Mycelial fluorescence was subsequently assessed under the microscope (scale bars indicate 5 µm). Flow cytometric analyses (FCA) is shown. The results are expressed as mean ± SD (standard deviation) of three independent biological replicates and were considered statistically different (*) when p<0.05. P-values were determined by a Student t test using GraphPad Prism software version 5 (GraphPad Software). DIC, differential interference contrast; FAU, fluorescent arbitrary units. (B) Oxygen consumption rates of the wild-type, ΔcnaA, alcA::pkcA and alcA::pkcA ΔcnaA strains when grown in minimal medium supplemented with 2% glycerol plus 100 mM threonine for 16 hours at 37°C.