Table 1. Characterisation of the non-stimulated CNS cell cultures and following an inflammatory stimulus.
Non-stimulated | LPS+IFN-γ stimulation | Fold change | ||
% O4+ cells | WT | 20.94±0.09 | 15.87±0.22 | 0.76±0.01 |
Hsp70.1 KO | 15.09±5.43 | 10.09±3.23 | 0.67±0.03 | |
% MBP+ cells | WT | 4.37±0.75 | 2.85±0.07 | 0.67±0.13 |
Hsp70.1 KO | 2.93±0.13 | 1.15±0.97 | 0.39±0.32 | |
% Casp3+ cells | WT | 1.80±0.63 | 4.01±1.66 | 2.51±0.86 |
Hsp70.1 KO | 2.31±0.58 | 3.57±0.63 | 1.60±0.33 | |
% O4+Casp3+ cells | WT | 0.51±0.43 | 2.19± 0.28 | 6.45±4.96 |
Hsp70.1 KO | 0.97±0.36 | 1.64± 0.58 | 1.70±0.13 |
The percentage of O4+, MBP+ and Casp3+ cells from the total number of cells of non-stimulated or LPS plus IFN-γ-stimulated cultures from wild-type (WT) and Hsp70.1 knock-out (KO) mice after 24 h. Fold change induced by inflammatory stimulus of each population was calculated referred to matched non-stimulated cultures. The results were expressed as the mean±SD.