Skip to main content
. 2014 Aug 25;9(8):e105479. doi: 10.1371/journal.pone.0105479

Figure 2. Inhibition of proteasome 26S resulted in an eNOS monomer accumulation in BAECs.

Figure 2

BAECs were incubated with indicated concentration of lactacystin overnight. The protein was harvested with RIPA buffer and run on SDS-PAGE gel at low temperature (4°C) and the eNOS monomer and dimer content was assessed by western blot and quantified using densitometry. (A) Lactacystin caused a dose-dependent accumulation of eNOS monomer but not dimer; (B) The eNOS monomer and dimer in BAECs were normalized with the controls (0 h) and represented as mean ± SE from four independent experiments. * P<0.05 comparison with control.