Table 2. Frontal cortex homocysteine, cysteine and glutathione concentrations (nmol/mg protein) after 2, 4 and 6 months of hyperhomocysteinemia induced by methionine treatment.
| Hcy (10−2 nmol/mg protein) | Cys (nmol/mg protein) | GSH (nmol/mg protein) | ||
| 2 months | CT (N = 8–10) | 4.78±0.9 | 5.20±0.8 | 6.15±0.6 |
| M05 (N = 9) | 10.03±1.1 * | 5.39±0.4 | 6.73±0.4 | |
| M1 (N = 6) | 9.07±2.1 | 4.68±0.6 | 5.21±1.0 | |
| p value (ANOVA) | 0.020 | 0.763 | 0.324 | |
| 4 months | CT (N = 9–10) | 9.07±1.9 | 5.50±0.7 | 5.14±0.5 |
| M05 (N = 8) | 10.30±2.2 | 6.54±0.7 | 4.13±0.5# | |
| M1 (N = 4–5) | 12.06±3.4 | 6.40±1.2 | 5.53±0.6 | |
| p value (ANOVA) | 0.480 | 0.582 | 0.323 | |
| 6 months | CT (N = 3) | 9.01±2.7 | 5.82±0.7 | 6.15±0.7 |
| M05 (N = 6) | 11.73±2.3 | 7.13±0.7 | 4.71±0.7# | |
| M1 (N = 7) | 10.50±2.0 | 6.67±0.3 | 5.94±0.3 | |
| p value (ANOVA) | 0.967 | 0.239 | 0.152 |
CT = control; M05 = Methionine 0.5%; M1 = Methionine 1%; Hcy = Homocysteine; Cys = Cysteine; GSH = Glutathione. Values presented as the mean ± SEM. * = Different from CT group in respective time of treatment (Tukey's post hoc test, p = 0.02). # = Different from M05 at 2 months (p<0.05)