Figure 5. DPM and IPP synthesis at high reactant concentration.

DPM and IPP were synthesized in separate, single-pot reactions. Reactions were initiated from acetate, or (R,S)-mevalonate. The conditions are described in Materials and Methods . Reactions initiated with acetate yielded 69% conversion of acetate to IPP (○), or 63% conversion of acetate to DPM (•). Reactions starting with (R,S)-mevalonate yielded 77% conversion of (R)-mevalonate to IPP (□), or 73% conversion of (R)-mevalonate to DPM (▪). The data points represent the average of results from three independent experiments.