Table 1. Enzymes used in the synthesis of DPM.

aEnzyme	EC #	Gene	Source	Substrate	d K m (mM)	d k cat (sec−1)
bACS	6.2.1.1	Acs1	S. cerevisiae	Acetate CoA	0.28 0.24	10
cACT	2.3.1.9	mvaE	E. faecalis	Ac-CoA	0.60	2.3
cHMGS2	2.3.3.10	mvaS	E. faecalis	acac-CoA Ac-CoA	0.015 0.35	1.0
cThRed	1.1.1.34	mvaE	E. faecalis	HMG-CoA	0.023	0.55
cHMGR	1.1.1.34	mvaE	E. faecalis	HMG-CoA	0.020	0.67
cMVK	2.7.1.36	mvaK1	S. aureus	Mev	0.027	19
cPMK	2.7.4.2	mvaK2	S. pneumoniae	P-mev	0.0042	5.0
bPK	2.7.1.40	PKM2	O. cuniculus	PEP	0.040	160
bMK	2.7.4.3	AK1	O. cuniculus	AMP	e0.50	e410
bPPiase	3.6.1.1	Ppa1	S. cerevisiae	PPi	e0.0050	e260

^aAbbreviations: ACS, acetyl-CoA synthetase; ACT, acetoacetyl-CoA thiolase; HMGS2, HMG-CoA synthase; ThRed, acetoacetyl-CoA thiolase/HMG-CoA reductase (dual-function enzyme); HMGR, HMG-CoA reductase; MVK, mevalonate kinase; PMK, phosphomevalonate kinase; PK, pyruvate kinase; MK, myokinase; PP_iase, inorganic pyrophosphatase.

^bObtained from commercial sources.

^cExpressed in E. coli and purified.

^dStandard errors are <5% in all cases (see Materials and Methods ).

^eParameters taken from literature (MK [77], [78], PP_iase [79], [80]).