. 2014 Aug 25;9(8):e105947. doi: 10.1371/journal.pone.0105947

Table 1. List of differentially expressed protein spots in influenza virus-infected and mock-infected MDCKs identified by MALDI-TOF/TOF.

Spot no.	Accession no.	Taxonomy	Description	Variation^a	Best ion score	Coverage (%)	Theoretical Mr/pI	Localization	Peptides identified
Cytoskeleton proteins
I26	gi\|296202808	Canis lupus familiaris	uncharacterized protein	↓	39	30%	33853/8.31	cytoplasm	SQYEQLAEQNRK
J11	gi\|359279916	Canis lupus familiaris	keratin 19	↑	53	56%	43858/5.16	cytoplasm	DAEAWFTSR
M10	gi\|57106334	Canis lupus familiaris	keratin, type I cytoskeletal 18 isoform 1	↓	29	35%	48209/5.33	cytoplasm	VKLEAEIATYR
N12	gi\|359323093	Canis lupus familiaris	keratin, type II cytoskeletal 8	↑	73	58%	54684/5.66	cytoplasm	SNIDNMFESYINNLR
N26	gi\|114667511	Canis lupus familiaris	keratin, type I cytoskeletal 10 isoform 3	↑	0	20%	58416/5.09	cytoplasm	GSLGGGFSSGGFSGG
Macromolecular biosynthesis
G11	gi\|119586801	Homo sapiens	heterogeneous nuclear ribonucleoprotein C (C1/C2), isoform CRA_c	↓	41	30%	19079/10.22	nucleus	GFAFVQYVNER
I17	gi\|57088769	Canis lupus familiaris	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1	↓	28	27%	31629/5.94	cytoplasm	AQGDEVDFAR
I19	gi\|50978728	Canis lupus familiaris	serine/threonine-protein phosphatase PP1-alpha catalytic subunit	↓	32	50%	38131/5.94	cytoplasm	ICGDIHGQYYDLLR
I20	gi\|168277712	Canis lupus familiaris	uncharacterized protein	↓	61	39%	48760/5.38	nucleus	NLPLPPPPPPR
I23	gi\|355712722	Canis lupus familiaris	uncharacterized protein	↓	55	42%	31507/4.79	nucleus	FIIENTDLAVANSIR
I28	gi\|209736954	Canis lupus familiaris	Nascent polypeptide-associated complex subunit alpha	↓	102	23%	23376/4.57	nucleus	DIELVMSQANVSR
I30	gi\|57086915	Canis lupus familiaris	protein DJ-1 isoform 1	↓	60	70%	20170/5.97	cytoplasm	GAEEMETVIPVDVMR
J2	gi\|168277712	Canis lupus familiaris	uncharacterized protein	↑	61	30%	48760/5.38	nucleus	NLPLPPPPPPR
J13	gi\|281338419	Canis lupus familiaris	Splicing factor	↑	34	61%	13350/10.12	nucleus	NPPGFAFVEFEDPR
J15	gi\|119574194	Homo sapiens	heterogeneous nuclear ribonucleoprotein H1 (H), isoform CRA_b	↑	98	27%	44025/6.47	nucleus	HTGPNSPDTANDGFVR
K20	gi\|210032390	Homo sapiens	protein SEC13 homolog isoform 2	↓	51	31%	34504/5.40	cytoplasm	NGGQILIADLR
M2	gi\|359323746	Canis lupus familiaris	T-complex protein 1 subunit epsilon isoform 1	↓	43	28%	60015/5.58	cytoplasm	WVGGPEIELIAIATGGR
M18	gi\|296207372	Canis lupus familiaris	uncharacterized protein	↓	45	22%	46415/4.90	nucleus	HPSKPDPSGECNPDLR
N18	gi\|355722466	Mustela putorius furo	serine/threonine kinase receptor associated protein	↑	87	47%	39094/5.54	nucleus	FSPDGELYASGSEDGTLR
N20	gi\|57088769	Canis lupus familiaris	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1	↑	28	27%	31629/5.94	cytoplasm	AQGDEVDFAR
N33	gi\|21465649	Canis lupus familiaris	Proteasome subunit beta type	↑	38	51%	22118/4.90	cytoplasm	LAAIAESGVER
Stress response
K1	gi\|296474454	Canis lupus familiaris	uncharacterized protein	↓	74	35%	78895/5.18	mitochondrion	HFSVEGQLEFR
K30	gi\|345803242	Canis lupus familiaris	peroxiredoxin-6	↓	107	60%	25052/5.71	lysosom	PGGLLLGDEAPNFEANTTIGR
K33	gi\|359322074	Canis lupus familiaris	peroxiredoxin-2-like isoform 1	↓	112	41%	22112/5.23	cytoplasm	EGGLGPLNIPLVADVTR
M25	gi\|33987931	Homo sapiens	HSP90AB1 protein	↓	50	27%	40270/4.89	mitochondrion	HFSVEGQLEFR
N3	gi\|345797614	Canis lupus familiaris	60 kDa heat shock protein, mitochondrial	↑	129	42%	61128/5.78	mitochondrion	AAVEEGIVLGGGCALLR
Signal transduction
I24	gi\|73946797	Canis lupus familiaris	annexin A1	↓	29	16%	38887/5.84	cytoplasm	TPAQFDADELR
I29	gi\|55742853	Canis lupus familiaris	annexin A4	↓	107	32%	36075/5.72	cytoplasm	GGTVKPASGFSATEDAQTLR
Metabolism associated proteins
J14	gi\|169403978	Canis lupus familiaris	glutathione peroxidase 1	↑	37	43%	22607/6.15	cytoplasm	FLVGPDGVPVR
N32	gi\|345789767	Canis lupus familiaris	inosine triphosphate pyrophosphatase	↑	21	51%	23485/6.17	cytoplasm	SAYALCTFAFSTGDPSEPVR
Viral proteins
J8	gi\|312436295	Influenza A virus	nonstructural protein 1 [Influenza A virus (A/Puerto Rico/8-CIP045_RG83841/1934(H1N1))]	↑	64	58%	25823/5.60	cytoplasm	VADQELGDAPFLDR
J9	gi\|299781726	Influenza A virus	nonstructural protein 1 [Influenza A virus (A/X-53(Puerto Rico/8/1934-New Jersey/11/1976)(H1N1))]	↑	87	62%	25508/6.70	cytoplasm	VADQELGDAPFLDR
J10	gi\|312436295	Influenza A virus	nonstructural protein 1 [Influenza A virus (A/Puerto Rico/8-CIP045_RG83841/1934(H1N1))]	↑	82	61%	25823/5.60	cytoplasm	VADQELGDAPFLDR
J12	gi\|312436295	Influenza A virus	nonstructural protein 1 [Influenza A virus (A/Puerto Rico/8-CIP045_RG83841/1934(H1N1))]	↑	56	54%	25823/5.60	cytoplasm	YLTDMTLEEMSR
N14	gi\|299781726	Influenza A virus (A/X-53(Puerto Rico/8/1934-New Jersey/11/1976)(H1N1))	nonstructural protein 1 [Influenza A virus (A/X-53(Puerto Rico/8/1934-New Jersey/11/1976)(H1N1))]	↑	87	62%	25508/6.70	cytoplasm	VADQELGDAPFLDR
Other proteins
I18	gi\|304373003	Canis lupus familiaris	uncharacterized protein	↓	69	44%	39642/5.54	extracelluar region	LKENTQHLNVEYER

The arrow“↑” represents the identified proteins were upregulated and the arrow “↓”represents the identified proteins were downregulated.