Fig. 5.
Complete drug study experimental protocol execution on the iMAP array. (A) HeLa based cytotoxicity assay validating the effect of paclitaxel, a well know anti-cancer agent that (B) reduces the growth and proliferation of cancer cells. (C) Study of the down regulation effects of the As2O3 drug on ESR1 in breast cancer cells MCF7. In a fully integrated on-chip assay the effects of arsenic trioxide on the protein expression of ESR1 in MCF7 cells were studied by loading 128 processing chambers with MCF7 and HeLa cells and stimulating the cells with several concentrations of As2O3 over a period of 72 h, after which the protein expression was analyzed on chip with immunofluorescent nuclear staining and detection. All standard error bars are based on the eight replicates (n = 8) in each processing module.