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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Cell Rep. 2014 Jul 31;8(4):1006–1017. doi: 10.1016/j.celrep.2014.06.063

Figure 5. General rules for postnatal axon-target matching and relationship to RGC birthdate.

Figure 5

(A-D) TRHR-RGC axons in dLGN at P5 (A), P8 (B), P15 (C), P20 (D). Scale = 250μm. (A’) Boxed region in A. Scale = 50μm. (E-H) TRHR-RGCaxons in the SC at P5 (A; asterisk: GFP+ meninges), P8 (B), P15 (C), P20 (D). Scale = 250μm. (I) Quantification of TRHR-RGC axon projections to the dLGN (light blue), OPN (magenta), NOT (orange), and SC (green); n=3-4 mice per age; ±SEMs. (J) Each RGC population examined here follows a general rule for axon-target matching: time of target innervation correlates with the number of incorrect targets transiently innervated during development. (K-S) Retinas from P8 mice injected with EdU at embryonic day 10 (E10). Cdh3-GFP mice (K-M), DRD4-GFP mice (N-P), Hoxd10-GFP mice (Q-S), GFP (top row) and EdU (middle row). Merge with Dapi (bottom row). Scale = 50μm. (T) GFP-expressing RGCs labeled with EdU at a given age of injection. ±SEM. (U) Age when 50% of the central (box) and peripheral (circle) GFP-expressing RGCs were born.