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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Neurobiol Learn Mem. 2014 May 10;114:70–80. doi: 10.1016/j.nlm.2014.04.012

Fig. 5.

Fig. 5

Expression of phosphorylated 2-amino-3-(3-hydroxy-5-methyl-isoxazol-4-yl)propanoic acid (AMPA) receptor subunit GluR 1 in the CA1 region. Seven- to nine-week old CD-1 wild-type or EAAT3 knockout mice were subjected to the fear conditioning stimuli. Their CA1 regions were harvested before the stimuli, 30 min or 180 min after the stimuli for Western blotting of phospho-GluR1 at S831 (panels A and C) or at S845 (panels B and D) in the plasma membrane (panels A and B) and in total cellular protein (panels C and D). A representative Western blot is shown in the top of each panel and a graphic presentation of the GluR1 protein abundance quantified by integrating the volume of autoradiogram bands from 6 to 16 mice for each experimental condition is shown in the bottom of each panel. Values in graphs are the means ± SEM. ^P < 0.05 compared with the corresponding value at time 0. EAAT3 −/− : glutamate transporter type 3 knockout.