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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Neurobiol Learn Mem. 2014 May 29;114:101–112. doi: 10.1016/j.nlm.2014.05.005

Figure 4.

Figure 4

Disruption of PKA anchoring in both CA3 and CA1 neurons, but not in CA1 neurons alone, impairs long-term contextual fear memory. A. Representative in situ hybridization images from sagittal brain sections demonstrated that both transgenic mouse lines expressed Ht31 in the major forebrain regions (upper panels). In the hippocampus, Ht31(1) mice expressed Ht31 throughout hippocampal subregions including the dentate gyrus, CA3, and CA1. Ht31(16) mice expressed Ht31 sparsely in CA3, but robustly in the dentate gyrus and CA1 (lower panels). B. Ht31(1) mice and wild-type littermates showed similar freezing levels during the retention test 1 hour after training (t-test, p = 0.076). Compared to wild-type littermates, Ht31(16) mice exhibited comparable freezing levels during the retention test 1 hour after training (t-test, p = 0.37). C. Ht31(1) mice displayed significantly decreased freezing levels during the retention test 24 hours after training relative to wild-type littermates (t-test, p = 0.033). However, Ht31(16) mice and wild-type littermates exhibited similar freezing levels during the retention test 24 hours after training (t-test, p = 0.452). * indicates p = 0.033. Error bars reflect S.E.M.