Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2015 Sep 1.

Published in final edited form as: Insect Biochem Mol Biol. 2014 Jun 12;52:23–32. doi: 10.1016/j.ibmb.2014.05.009

Fig. 2 — AaMet is required for JH III induction of AaKr-h1 gene.

A. Aag-2 cells were incubated with AaMet or AaKr-h1 dsRNA for 72 hr. Then the cells were exposed to 1 μM of JH III for 2 hr. Total RNAs were isolated and used in qRT-PCR to quantify AaMet and AaKr-h1 mRNA levels. AaS7RP mRNA levels were used for normalization. Mean + S.E. (n=3) are shown.

B. Aag-2 cells were transfected with empty pIEx-4 expression plasmid and this plasmid containing complete ORF of AaMet. Three days after transfection, the cells were exposed to 1 μM of JH III for 2 hr. Total RNAs were isolated and used in qRT-PCR to quantify AaMet and AaKr-h1 mRNA levels. AaS7RP mRNA levels were used for normalization. Mean + S.E. (n=3) are shown.

C. Aag-2 cells were incubated with AaMet or AaKr-h1 dsRNA targeting 5’ UTR for 72 hr. Then the cells were transfected with pIEx-4 AaMet expression plasmid. Three days after transfection, the cells were exposed to 1 μM of JH III for 2 hr. Total RNAs were isolated and used in qRT-PCR to quantify AaMet and AaKr-h1 mRNA levels. AaS7RP mRNA levels were used for normalization. Mean + S.E. (n=3) are shown.

The data were analyzed using Univariate analysis of variance Post Hoc Tests. **, significantly different at P<0.01; *, significantly different at P<0.05.

Fig. 2 — AaMet is required for JH III induction of AaKr-h1 gene.

A. Aag-2 cells were incubated with AaMet or AaKr-h1 dsRNA for 72 hr. Then the cells were exposed to 1 μM of JH III for 2 hr. Total RNAs were isolated and used in qRT-PCR to quantify AaMet and AaKr-h1 mRNA levels. AaS7RP mRNA levels were used for normalization. Mean + S.E. (n=3) are shown.

B. Aag-2 cells were transfected with empty pIEx-4 expression plasmid and this plasmid containing complete ORF of AaMet. Three days after transfection, the cells were exposed to 1 μM of JH III for 2 hr. Total RNAs were isolated and used in qRT-PCR to quantify AaMet and AaKr-h1 mRNA levels. AaS7RP mRNA levels were used for normalization. Mean + S.E. (n=3) are shown.

C. Aag-2 cells were incubated with AaMet or AaKr-h1 dsRNA targeting 5’ UTR for 72 hr. Then the cells were transfected with pIEx-4 AaMet expression plasmid. Three days after transfection, the cells were exposed to 1 μM of JH III for 2 hr. Total RNAs were isolated and used in qRT-PCR to quantify AaMet and AaKr-h1 mRNA levels. AaS7RP mRNA levels were used for normalization. Mean + S.E. (n=3) are shown.

The data were analyzed using Univariate analysis of variance Post Hoc Tests. **, significantly different at P<0.01; *, significantly different at P<0.05.