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. Author manuscript; available in PMC: 2014 Aug 25.
Published in final edited form as: Cytometry A. 2014 Apr 3;85(6):548–555. doi: 10.1002/cyto.a.22434

Figure 5. Effects of DiD on cell proliferation, migration, and apoptosis.

Figure 5

To determine possible adverse effects of DiD on cell function, PCa cells (PC3, DU145, C42B) were stained with DiD (0.5 μM and 2 μM), and a selection of functional assays were performed. (A) Cell proliferation was measured by MTS assay. (B) Cell migration was assessed using a wound-healing assay. Original magnification, 10x / 0.25 Ph1 ADL. Scale bars: 250 μm. After 2 days of DiD staining, (C) apoptosis was analyzed by flow cytometry with Annexin V staining and (D) the number of dead cells was counted by trypan blue staining. Data are presented as mean ± standard deviation from triplicate determinations.