Table 1. Quantification of RC, cytbc1, ATP synthase and terminal cytc oxidases within chromatophores by mass spectrometry.
Protein | Tryptic peptide | Quantity (nmol/g total protein) |
Stoichio- metry (ratio per 1 PufM) |
Stoichio- metry (ratio per 24 PufM) |
---|---|---|---|---|
RC PufM | AEYQNIFSQVQVR | 724 ± 1 | 1.00 ± 0.00 | 24.0 ± 0.0 |
RC PufL | ALLSFER | 778 ± 112 | 1.07 ± 0.15 | 25.8 ± 3.7 |
Cytbc1 FbcC | SLSEPGGPELPEDQVR | 257 ± 3 | 0.36 ± 0.00 | 8.5 ± 0.1 |
Cytbc1 FbcF | SVQLGQLVDTNAR | 203 ± 14 | 0.28 ± 0.02 | 6.7 ± 0.5 |
ATP synthase AtpF | LAAAEDQIASAEAGAVR | 65 ± 9 | 0.09 ± 0.01 | 2.1 ± 0.3 |
ATP synthase AtpX | SDAAAVDAAVAAR | 61 ± 2 | 0.08 ± 0.00 | 2.0 ± 0.1 |
ATP synthase AtpA | GIQAAEISAILK | 134 ± 6 | 0.18 ± 0.01 | 4.4 ± 0.2 |
VVDGLGNPIDGK | 143 ± 9 | 0.20 ± 0.01 | 4.8 ± 0.2 | |
TAIALDTILNQK | 143 ± 1 | 0.20 ± 0.00 | 4.7 ± 0.0 | |
Cytc oxidase CoxII | VVSEEAYAAWLEQAR | 0 ± 0 | 0.00 ± 0.00 | 0.0 ± 0.0 |
Cytc oxidase CcoO | AQANPDADTDGLLER | 31 ± 0 | 0.04 ± 0.00 | 1.0 ± 0.0 |