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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Jul 8;52:102–114. doi: 10.1016/j.ibmb.2014.06.010

Fig. 8. RNAi knockdown of AsCTLs and survival of Ar. subalbatus females after bacterial infection.

Fig. 8

Newly emerged mosquito females were injected with dsRNA to each AsCTL or GFP (as a control), and expression of AsCTLs transcripts in the dsRNA-injected mosquitoes was determined by real-time PCR four days after dsRNA injection (A–C). Each bar represents the mean of three individual measurements ± SEM.“**” indicates significant difference in the levels of mRNAs between the control (GFP-RNAi) and AsCTL-RNAi groups determined by the unpaired t-test. Four days after dsRNA injection, the mosquitoes were injected with live E. coli (D–F) or live M. luteus (G–I), and the survival of mosquitoes was recorded for 7 days after bacteria injection. The survival curves were generated by Kaplan-Meier method and the statistical analysis was performed by the log rank test (p<0.05 indicates significant difference).