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Nestin depletion and DNA synthesis. (Panel A) Aortic VSMCs infected with a lentivirus containing a shRNAmir directed against nestin reduced protein expression, whereas smooth muscle α-actin (SMA), caldesmon and smooth muscle-22α protein levels were unchanged. (Panel B) In nestin-depleted aortic VSMCs, 3H-thymidine uptake was significantly decreased. (*) Denotes P < 0.05 versus VSMCs infected with the empty lentivirus.
