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. 2014 Aug 21;13:119. doi: 10.1186/s12933-014-0119-6

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© Tardif et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Nestin ⁽⁺⁾ -VSMCs were actively involved in the cell cycle; impact of type I diabetes. A subpopulation of nestin⁽⁺⁾-VSMCs and nestin⁽⁺⁾-endothelial cells in the (Panel A) carotid artery and (Panel C) aorta arch of normal adult rats were associated with nuclear PHH3 staining. In type I diabetic rats, the subpopulation of nestin⁽⁺⁾-VSMCs co-expressing nuclear PHH3 staining in the (Panel B) carotid artery and (Panel D) aortic arch was reduced and nestin/PHH3 co-staining of endothelial cells was also diminished. The arrow indicates the luminal side and the nucleus was identified by DAPI (blue fluorescence).