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. 2014 Aug 15;7:372. doi: 10.1186/1756-3305-7-372

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© Zhang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Pro-inflammatory cytokines (IL-1 β, IL-6, and TNF-α) produced by BV-2 cells. Mouse microglia BV-2 was either left uninfected, or T.gondii-infected (T.gondii) or T.gondii-infected and treated with minocycline (T.gondii + M) as described in the Methods section. (A) The mRNAs of pro-inflammatory cytokines were detected by quantitative RT-PCR. The relative levels of IL-1 β, IL-6, and TNF-α expression were compared with GAPDH. (B) Culture supernatants were collected after 24 h post-treatment and tested by ELISA to quantify IL-1 β, IL-6, and TNF-α secretion. The results represent the means ± SEM of three independent experiments. (*P < 0.01 vs Control; #P < 0.01 vs T. gondii).